Construction of an ordered cosmid collection of the Escherichia coli K-12 W3110 chromosome

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Construction of an ordered cosmid collection of the Escherichia coli K-12 W3110 chromosome.

A cosmid library of the Escherichia coli K-12 W3110 chromosome was constructed in which clones were assigned to locations on the chromosome map by hybridization and genetic marker complementation tests. Approximately 70% of the genome was represented by this library. The identified clones can be maintained in the homologous system and would facilitate genetic studies of E. coli.

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Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110

With the goal of solving the whole-cell problem with Escherichia coli K-12 as a model cell, highly accurate genomes were determined for two closely related K-12 strains, MG1655 and W3110. Completion of the W3110 genome and comparison with the MG1655 genome revealed differences at 267 sites, including 251 sites with short, mostly single-nucleotide, insertions or deletions (indels) or base substi...

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Cosmid cloning of Rickettsia prowazekii antigens in Escherichia coli K-12.

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ژورنال

عنوان ژورنال: Journal of Bacteriology

سال: 1989

ISSN: 0021-9193,1098-5530

DOI: 10.1128/jb.171.2.1214-1218.1989